Publications
Group highlights
At the end of this page, you can find the full list of publications. New papers will be available on bioRxiv.
Natural DNA is right-handed — what happens if you use left-handed DNA instead? We show that L-DNA-PAINT preserves the same specificity and multiplexing capabilities as R-DNA-PAINT, while enhancing nuclear target imaging by significantly reducing background signal.
Hylkje J. Geertsema, Giulia Aimola, Valentin Fabricius, Jens P. Fürste, Benedikt B. Kaufer, Helge Ewers
Nature Biotechnology 39, 551-554 (2021)
See News and Views by Rita Strack.
Full List of publications
[PREPRINT] High-throughput single molecule microscopy with adaptable spatial resolution using exchangeable oligonucleotide labels
Klarinda de Zwaan, Ran Huo, Myron N.F. Hensgens, Hannah Lena Wienecke, Miyase Tekpinar, Hylkje J. Geertsema, Kristin S. Grußmayer
preprint
Unblending of Transcriptional Condensates in Human Repeat Expansion Disease
Shaon Basu, Sebastian D. Mackowiak, Henri Niskanen, Dora Knezevic, Vahid Asimi, Stefanie Grosswendt, Hylkje J. Geertsema, Salaheddine Ali, Ivana Jerković, Helge Ewers, Stefan Mundlos, Alexander Meissner, Daniel M. Ibrahmi, Denes Hnisz
Cell, Volume 181, Issue 5, 1062-1079.e30
Left-handed DNA-PAINT for improved super-resolution imaging in the nucleus
Hylkje J. Geertsema, Giulia Aimola, Valentin Fabricius, Jens P. Fürste, Benedikt B. Kaufer, Helge Ewers
Nature Biotechnology 39, 551-554 (2021)
Rapid and efficient C-terminal labeling of nanobodies for DNA-PAINT
Valentin Fabricius, Jonathan Lefèbre, Hylkje J. Geertsema, Stephen F. Marino, Helge Ewers
J. Phys. D: Appl. Phys. 51 474005 (2018)
Simultaneous Real-Time Imaging of Leading and Lagging Strand Synthesis Reveals the Coordination Dynamics of Single Replisomes
Karl E. Duderstadt, Hylkje J. Geertsema, Sarah A. Stratmann, Christiaan M. Punter, Arkadiusz W. Kulczyk, Charles C. Richardson, Antoine M. van Oijen
Molecular Cell, Volume 64, Issue 6, 1035 - 1047 (2016)
Single-Molecule Observation of Prokaryotic DNA Replication
Hylkje J. Geertsema, Karl E. Duderstadt, Antoine M. van Oijen
Book chapter in Methods in Molecular Biology (2015)
Single-Molecule Imaging at High Fluorophore Concentrations by Local Activation of Dye
Hylkje J. Geertsema, Aartje C. Schulte, Lisanne M. Spenkelink, William J. McGrath, Seamus R. Morrone, Jungsan Sohn, Walter F. Mangel, Andrew Robinson, Antoine M. van Oijen
Biophysical Journal, Volume 108, Issue 4, 949 - 956 (2015)
Single-molecule studies of polymerase dynamics and stoichiometry at the bacteriophage T7 replication machinery
Hylkje J. Geertsema, Arkadiusz W. Kulczyk, Charles C. Richardson, Antoine M. van Oijen
Proceedings of the National Academy of Sciences, Volume 111, No. 11, 4073-4078 (2014)
A single-molecule view of DNA replication: the dynamic nature of multi-protein complexes revealed
Hylkje J. Geertsema, Antoine M. van Oijen
Current Opinion in Structural Biology, Volume 23, Issue 5, 788-793 (2013)